Rapid and timely diagnosis of infectious diseases at the point-of-care is critical for prompt and appropriate therapeutic intervention. There is an urgent need for inexpensive, point-of-care, molecular diagnostic systems in low and middle-income countries due to lack of testing facilities, shortage of trained personnel, and severe cost constraints. In this talk, I will present our work towards the development of inexpensive, microfluidic-based systems for infectious disease diagnostics and therapy monitoring such as HIV viral load testing. The main focus of my talk will be on: 1) Disposable plasma separators for relatively large-volume plasma separation without a need for any centrifugation; 2) a ‘nuclemeter’ lab-on-chip device based on a reaction-diffusion mode amplification and fluorescent detection for simplified endpoint quantitative detection; and 3) Smart cup for non-instrumented, smartphone-based molecular diagnostics. These devices are suitable for use at home, in the field, and in poor-resource settings, where access to sophisticated laboratories is impractical, unaffordable, or nonexistent.